negative selection kit (R&D Systems)

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R&D Systems negative selection kit
Negative Selection Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magcellect+human+nk+cell+isolation+kit/MagCellect+Human+NK+Cell+Isolation+Kit/pm33928707-23-11-20
Average 91 stars, based on 3 article reviews

negative selection kit - by Bioz Stars, 2026-09

91/100 stars

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Isolation:

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: .. PB was taken from healthy volunteers, and PB NK cells, isolated from total mononuclear cells, separated after centrifugation on Ficoll-Paque Plus (GE Healthcare Life Sciences) for 30 min at 400 g . PB NK cells were isolated by use of a MagCellect Human NK Cell Isolation kit (R&D Systems), according to the manufacturer’s instructions. ..

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: .. PB was taken from healthy volunteers, and PB NK cells, isolated from total mononuclear cells, separated after centrifugation on Ficoll-Paque Plus (GE Healthcare Life Sciences) for 30 min at 400 g. PB NK cells were isolated by use of a MagCellect Human NK Cell Isolation kit (R&D Systems), according to the manufacturer’s instructions. ..

Article Title: The phenotype of decidual CD56+ lymphocytes is influenced by secreted factors from decidual stromal cells but not macrophages in the first trimester of pregnancy.
Article Snippet: .. In brief, non-adhered immune cells, containing the dNK cell fraction, from DSC isolation were purified by use of negative selection with a MagCellect Human NK Cell Isolation Kit (R&D Systems, Abingdon, UK), according to manufacturer’s instructions. .. Purity, as measured by CD56+ cells, was 95.26 %±0.73 % (mean± SEM, n = 19), and viability, immediately upon isolation, was 87.3 %±1.86 % (mean± SEM, n = 19), as assessed by fixable viability dye (eBioscience, Hatfield, UK).

Centrifugation:

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: .. PB was taken from healthy volunteers, and PB NK cells, isolated from total mononuclear cells, separated after centrifugation on Ficoll-Paque Plus (GE Healthcare Life Sciences) for 30 min at 400 g . PB NK cells were isolated by use of a MagCellect Human NK Cell Isolation kit (R&D Systems), according to the manufacturer’s instructions. ..

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: .. PB was taken from healthy volunteers, and PB NK cells, isolated from total mononuclear cells, separated after centrifugation on Ficoll-Paque Plus (GE Healthcare Life Sciences) for 30 min at 400 g. PB NK cells were isolated by use of a MagCellect Human NK Cell Isolation kit (R&D Systems), according to the manufacturer’s instructions. ..

Cell Isolation:

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: .. PB was taken from healthy volunteers, and PB NK cells, isolated from total mononuclear cells, separated after centrifugation on Ficoll-Paque Plus (GE Healthcare Life Sciences) for 30 min at 400 g . PB NK cells were isolated by use of a MagCellect Human NK Cell Isolation kit (R&D Systems), according to the manufacturer’s instructions. ..

Article Title: Oxygen Modulates Human Decidual Natural Killer Cell Surface Receptor Expression and Interactions with Trophoblasts
Article Snippet: Resultant tissue digest was passed sequentially through 100-μm and 70-μm filters and layered onto Ficoll-Paque (GE Healthcare Life Sciences, Buckinghamshire, U.K.). .. The buffy layer was collected, and cells were resuspended in 10 ml of dNK cell culture medium (phenol red-free RPMI 1640 medium supplemented with 10% [v/v] fetal bovine serum [FBS] containing 2 mmol/L L-glutamine, 100 IU/ml penicillin, 100 μg/ml streptomycin, and 2.5 μg/ml amphotericin) and plated on cell culture dishes in a 37°C incubator for 15 min. Nonadherent cells containing the dNK cell fraction were then purified by negative selection using a MagCellect human NK cell isolation kit (R&D Systems, Abingdon, U.K.) according to the manufacturer's instructions. .. Purity of cells as measured by CD56-positivity was, on average, 95.7 ± 0.92%, and viability immediately upon isolation was 96.5 ± 0.38% (n = 33), as assessed by fixable viability dye (eBioscience, Hatfield, U.K.).

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: The resultant tissue digest was passed sequentially through 100 and 70 mm filters and layered onto Ficoll-Paque (GE Healthcare Life Sciences, Buckinghamshire, United Kingdom). .. The buffy layer was collected, and cells were resuspended in 10 ml dNK cell-culture media [Phenol Red Free RPMI 1640, supplemented with 10% (v/v) FBS, containing 2 mmol/L L-glutamine, 100 IU/ml penicillin, 100 mg/ml streptomycin, and 2.5 mg/ml amphotericin] and plated in a 37°C incubator for 15 min. Nonadherent cells, containing the dNK cell fraction, were purified by use of negative selection with a MagCellect Human NK Cell Isolation Kit (R&D Systems, Abingdon, United Kingdom), according to the manufacturer’s instructions. .. Purity, as measured by CD56+ cells, was, on average, 95.7 6 0.92% (n = 33), and viability, immediately upon isolation, was 96.5 6 0.38% (n = 33), as assessed by fixable viability dye (eBioscience, Hatfield, United Kingdom).

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: The resultant tissue digest was passed sequentially through 100 and 70 μ m filters and layered onto Ficoll-Paque (GE Healthcare Life Sciences, Buckinghamshire, United Kingdom). .. The buffy layer was collected, and cells were resuspended in 10 ml dNK cell-culture media [Phenol Red Free RPMI 1640, supplemented with 10% (v/v) FBS, containing 2 mmol/L L-glutamine, 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 2.5 μ g/ml amphotericin] and plated in a 37°C incubator for 15 min. Nonadherent cells, containing the dNK cell fraction, were purified by use of negative selection with a MagCellect Human NK Cell Isolation Kit (R&D Systems, Abingdon, United Kingdom), according to the manufacturer’s instructions. .. Purity, as measured by CD56+ cells, was, on average, 95.7 ± 0.92% ( n = 33), and viability, immediately upon isolation, was 96.5 ± 0.38% ( n = 33), as assessed by fixable viability dye (eBioscience, Hatfield, United Kingdom).

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: .. PB was taken from healthy volunteers, and PB NK cells, isolated from total mononuclear cells, separated after centrifugation on Ficoll-Paque Plus (GE Healthcare Life Sciences) for 30 min at 400 g. PB NK cells were isolated by use of a MagCellect Human NK Cell Isolation kit (R&D Systems), according to the manufacturer’s instructions. ..

Cell Culture:

Purification:

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: The resultant tissue digest was passed sequentially through 100 and 70 mm filters and layered onto Ficoll-Paque (GE Healthcare Life Sciences, Buckinghamshire, United Kingdom). .. The buffy layer was collected, and cells were resuspended in 10 ml dNK cell-culture media [Phenol Red Free RPMI 1640, supplemented with 10% (v/v) FBS, containing 2 mmol/L L-glutamine, 100 IU/ml penicillin, 100 mg/ml streptomycin, and 2.5 mg/ml amphotericin] and plated in a 37°C incubator for 15 min. Nonadherent cells, containing the dNK cell fraction, were purified by use of negative selection with a MagCellect Human NK Cell Isolation Kit (R&D Systems, Abingdon, United Kingdom), according to the manufacturer’s instructions. .. Purity, as measured by CD56+ cells, was, on average, 95.7 6 0.92% (n = 33), and viability, immediately upon isolation, was 96.5 6 0.38% (n = 33), as assessed by fixable viability dye (eBioscience, Hatfield, United Kingdom).

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: The resultant tissue digest was passed sequentially through 100 and 70 μ m filters and layered onto Ficoll-Paque (GE Healthcare Life Sciences, Buckinghamshire, United Kingdom). .. The buffy layer was collected, and cells were resuspended in 10 ml dNK cell-culture media [Phenol Red Free RPMI 1640, supplemented with 10% (v/v) FBS, containing 2 mmol/L L-glutamine, 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 2.5 μ g/ml amphotericin] and plated in a 37°C incubator for 15 min. Nonadherent cells, containing the dNK cell fraction, were purified by use of negative selection with a MagCellect Human NK Cell Isolation Kit (R&D Systems, Abingdon, United Kingdom), according to the manufacturer’s instructions. .. Purity, as measured by CD56+ cells, was, on average, 95.7 ± 0.92% ( n = 33), and viability, immediately upon isolation, was 96.5 ± 0.38% ( n = 33), as assessed by fixable viability dye (eBioscience, Hatfield, United Kingdom).

Selection:

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: The resultant tissue digest was passed sequentially through 100 and 70 mm filters and layered onto Ficoll-Paque (GE Healthcare Life Sciences, Buckinghamshire, United Kingdom). .. The buffy layer was collected, and cells were resuspended in 10 ml dNK cell-culture media [Phenol Red Free RPMI 1640, supplemented with 10% (v/v) FBS, containing 2 mmol/L L-glutamine, 100 IU/ml penicillin, 100 mg/ml streptomycin, and 2.5 mg/ml amphotericin] and plated in a 37°C incubator for 15 min. Nonadherent cells, containing the dNK cell fraction, were purified by use of negative selection with a MagCellect Human NK Cell Isolation Kit (R&D Systems, Abingdon, United Kingdom), according to the manufacturer’s instructions. .. Purity, as measured by CD56+ cells, was, on average, 95.7 6 0.92% (n = 33), and viability, immediately upon isolation, was 96.5 6 0.38% (n = 33), as assessed by fixable viability dye (eBioscience, Hatfield, United Kingdom).

Article Title: Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia
Article Snippet: The resultant tissue digest was passed sequentially through 100 and 70 μ m filters and layered onto Ficoll-Paque (GE Healthcare Life Sciences, Buckinghamshire, United Kingdom). .. The buffy layer was collected, and cells were resuspended in 10 ml dNK cell-culture media [Phenol Red Free RPMI 1640, supplemented with 10% (v/v) FBS, containing 2 mmol/L L-glutamine, 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 2.5 μ g/ml amphotericin] and plated in a 37°C incubator for 15 min. Nonadherent cells, containing the dNK cell fraction, were purified by use of negative selection with a MagCellect Human NK Cell Isolation Kit (R&D Systems, Abingdon, United Kingdom), according to the manufacturer’s instructions. .. Purity, as measured by CD56+ cells, was, on average, 95.7 ± 0.92% ( n = 33), and viability, immediately upon isolation, was 96.5 ± 0.38% ( n = 33), as assessed by fixable viability dye (eBioscience, Hatfield, United Kingdom).

other:

Article Title: Oxygen Modulates Human Decidual Natural Killer Cell Surface Receptor Expression and Interactions with Trophoblasts
Article Snippet: Purity of cells as measured by CD56-positivity was, on average, 95.7 ± 0.92%, and viability immediately upon isolation was 96.5 ± 0.38% (n = 33), as assessed by fixable viability dye (eBioscience, Hatfield, U.K.).

Review